Biochemical and morphological comparison of two tumour-cell-aggregation factors from rat ascites hepatoma cells
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چکیده
منابع مشابه
Roles of calcium in aggregation of rat ascites hepatoma cells by cell surface-associated adhesive factor.
Our previous studies have shown that a cell surface-associated adhesive factor (AF), separated from rat ascites hepatoma AH136B cells of a differentiated type and highly purified by chromatography, induces not only aggregation of dissociated AH136B cells or rat ascites hepatoma AH109A cells of an undifferentiated type but also adhesiveness characterized by the development of junctional complexe...
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protein tyrosine phosphatases (ptpases) regulate tyrosine phosphorylation of target proteins involved in several aspects of cellular functions. enzyme activities of the ptpases in cytosolic and particulate fractions of rat ascites hepatoma cell lines were determined and compared with those of normal rat liver. our present data revealed that although there was no neoplatic-specific alteration of...
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Rates of histone acetylation and deacetylation in nuclei from fetal, adult, and two kinds of neoplastic rat hepatocytes were examined. Histone acetylation in isolated nuclei was measured in the presence of 6 mM sodium n-butyrate, a potent inhibitor of deacetylase, and in the absence of the inhibitor. The deacetylase activity was estimated from the difference between the rates with or without th...
متن کاملComparison of nucleolar proteins of normal rat liver and Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis.
A two-dimensional polyacrylamide gel electrophoresis technique is presented for separation of acid-extracted proteins of isolated nucleoli of normal rat liver and Novikoff hepatoma ascites cells. About 100 distinct protein spots were resolved. Comparison of the patterns for normal liver and Novikoff hepatoma revealed nine spots with markedly different intensities in the two patterns. Two spots ...
متن کاملMacrophage potentiation of invasive capacity of rat ascites hepatoma cells.
In vitro and in vivo invasive capacities of rat ascites hepatoma cells (AH 130) that had been cultured on the feeder layers of rat macrophages were examined. The in vitro invasive capacity of the tumor cells was measured by their ability to form tumor cell colonies underneath cultured mesothelial cell monolayers; in vivo invasive capacity was examined by the implantation of the tumor cells into...
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ژورنال
عنوان ژورنال: British Journal of Cancer
سال: 1978
ISSN: 0007-0920,1532-1827
DOI: 10.1038/bjc.1978.82